MersV1, Main, Exploration, bibRecord, 003C40

Specific inhibition of in vitro reverse transcription using antisense oligonucleotides targeted to the TAR regions of HIV-1 and HIV-2

Identifieur interne : 003C40 ( Main/Exploration ); précédent : 003C39; suivant : 003C41

Specific inhibition of in vitro reverse transcription using antisense oligonucleotides targeted to the TAR regions of HIV-1 and HIV-2

Auteurs : Florence Boulme [France] ; Maritta Per L Heape [France] ; Leila Sarih-Cottin [France] ; Simon Litvak [France]

Source :

BBA - Gene Structure and Expression [ 0167-4781 ] ; 1997.

RBID : ISTEX:191164A3CCA1C0429CDAB785CEE915EA596FE97E

English descriptors

Teeft :
- Acta, Antisense, Antisense odns, Antisense oligonucleotides, Biochimica, Biol, Biophysica, Biophysica acta, Blockage, Boulme, Cdna, Cdna synthesis, Control odns, Important role, Nucleic acids, Odns, Oligonucleotides, Primer, Replication, Retroviral, Rnase, Secondary structure, Target sequence, Transcription, Viral.

Abstract

Abstract: Antisense oligonucleotides (ODNs) overlapping the stem-loop structure of the trans-activating responsive (TAR) element at the 5′ end of HIV-1 and HIV-2 viral RNAs were tested for their inhibitory effect on cDNA synthesis by HIV-1 and HIV-2 reverse transcriptases (RT). Inhibition of reverse transcription is sequence-specific and enhanced by the presence of the RT-associated RNase H activity. The degree of inhibition obtained with the anti-TAR antisense is significantly higher than with other HIV-1 targeted antisense ODNs used before [1]. Gel retardation showed a stable specific complex between the 16- and 25-mer anti-TAR HIV-1 selected ODNs and the target region. No complex was observed with a non-inhibitor 22-mer anti-TAR ODN and with the corresponding control sequences. Targeting of the first stem-loop in the 5′ region of HIV-2 RNA by anti-TAR ODNs inhibited very strongly reverse transcription by HIV-2 RT. The structure of the antisense and the target sequence affect annealing efficiency and hence the degree of inhibition of reverse transcription. © 1997 Elsevier Science B.V. All rights reserved.

Url:

https://api.istex.fr/ark:/67375/6H6-06V08SB7-Z/fulltext.pdf

DOI: 10.1016/S0167-4781(97)00026-2

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Antisense oligonucleotides (ODNs) overlapping the stem-loop structure of the trans-activating responsive (TAR) element at the 5′ end of HIV-1 and HIV-2 viral RNAs were tested for their inhibitory effect on cDNA synthesis by HIV-1 and HIV-2 reverse transcriptases (RT). Inhibition of reverse transcription is sequence-specific and enhanced by the presence of the RT-associated RNase H activity. The degree of inhibition obtained with the anti-TAR antisense is significantly higher than with other HIV-1 targeted antisense ODNs used before [1]. Gel retardation showed a stable specific complex between the 16- and 25-mer anti-TAR HIV-1 selected ODNs and the target region. No complex was observed with a non-inhibitor 22-mer anti-TAR ODN and with the corresponding control sequences. Targeting of the first stem-loop in the 5′ region of HIV-2 RNA by anti-TAR ODNs inhibited very strongly reverse transcription by HIV-2 RT. The structure of the antisense and the target sequence affect annealing efficiency and hence the degree of inhibition of reverse transcription. © 1997 Elsevier Science B.V. All rights reserved.</div>
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Specific inhibition of in vitro reverse transcription using antisense oligonucleotides targeted to the TAR regions of HIV-1 and HIV-2

Specific inhibition of in vitro reverse transcription using antisense oligonucleotides targeted to the TAR regions of HIV-1 and HIV-2

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri